AP1B1 Antibody

ProSci
Product Code: PSI-16-538
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-16-538-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using AP1B1 antibody (16-538) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5min.

Western blot analysis of extracts of various cell lines, using AP1B1 antibody (16-538) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5min.

Further Information

Additional Names:
ADTB1, AP105A, BAM22, CLAPB2, AP-1 complex subunit beta-1, ADTB1, CLAPB2, Golgi adaptor HA1/AP1 adaptin beta subunit, adapter-related protein complex 1 subunit beta-1, adaptor protein complex AP-1 subunit beta-1, adaptor related protein complex 1 beta 1 subunit, beta-1-adaptin, beta-adaptin 1, beta-prime-adaptin, beta1-adaptin, clathrin assembly protein complex 1 beta large chain, plasma membrane adaptor HA2/AP2 adaptor beta subunit
Application Note:
WB: 1:500 - 1:2000
Background:
Adaptor protein complex 1 is found at the cytoplasmic face of coated vesicles located at the Golgi complex, where it mediates both the recruitment of clathrin to the membrane and the recognition of sorting signals within the cytosolic tails of transmembrane receptors. This complex is a heterotetramer composed of two large, one medium, and one small adaptin subunit. The protein encoded by this gene serves as one of the large subunits of this complex and is a member of the adaptin protein family. This gene is a candidate meningioma gene. Alternative splicing results in multiple transcript variants.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 700-800 of human AP1B1 (NP_001118.3).
NCBI Gene ID #:
162
NCBI Official Name:
adaptor related protein complex 1 subunit beta 1
NCBI Official Symbol:
AP1B1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 105kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
Q10567
User NOte:
Optimal dilutions for each application to be determined by the researcher.