NUBP1 Antibody

ProSci
Product Code: PSI-16-590
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-16-590-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of HepG2 cells, using NUBP1 antibody (16-590) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
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Immunohistochemistry of paraffin-embedded rat heart using NUBP1 antibody (16-590) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human thyroid cancer using NUBP1 antibody (16-590) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human colon carcinoma using NUBP1 antibody (16-590) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse liver using NUBP1 antibody (16-590) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse kidney using NUBP1 antibody (16-590) at dilution of 1:100 (40x lens).

Western blot analysis of extracts of HepG2 cells, using NUBP1 antibody (16-590) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
Immunohistochemistry of paraffin-embedded rat heart using NUBP1 antibody (16-590) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human thyroid cancer using NUBP1 antibody (16-590) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using NUBP1 antibody (16-590) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using NUBP1 antibody (16-590) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using NUBP1 antibody (16-590) at dilution of 1:100 (40x lens).

Further Information

Additional Names:
Cytosolic Fe-S cluster assembly factor NUBP1 {ECO:0000255|HAMAP-Rule:MF_03038}, Nucleotide-binding protein 1 {ECO:0000255|HAMAP-Rule:MF_03038}, NBP 1 {ECO:0000255|HAMAP-Rule:MF_03038}, NUBP1 {ECO:0000255|HAMAP-Rule:MF_03038}, NBP, NBP1
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200
Background:
NUBP1 is a member of the NUBP/MRP subfamily of ATP-binding proteins.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 201-320 of human NUBP1 (NP_002475.2).
NCBI Gene ID #:
4682
NCBI Official Name:
Cytosolic Fe-S cluster assembly factor NUBP1 {ECO:0000255|HAMAP-Rule:MF_03038}
NCBI Official Symbol:
NUBP1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 38kDa
Purification:
Affinity purification
Research Area:
Cell Cycle
Swissprot #:
P53384
User NOte:
Optimal dilutions for each application to be determined by the researcher.