Heparanase 1 Antibody

ProSci
Product Code: PSI-16-627
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-16-627-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cells, using Heparanase 1 antibody (16-627) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
2 / 3
Immunofluorescence analysis of HeLa cells using Heparanase 1 antibody (16-627) at dilution of 1:100. Blue: DAPI for nuclear staining.
3 / 3
Immunofluorescence analysis of NIH/3T3 cells using Heparanase 1 antibody (16-627) at dilution of 1:100. Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cells, using Heparanase 1 antibody (16-627) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunofluorescence analysis of HeLa cells using Heparanase 1 antibody (16-627) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using Heparanase 1 antibody (16-627) at dilution of 1:100. Blue: DAPI for nuclear staining.

Further Information

Additional Names:
HPSE, Endo-glucoronidase, Heparanase-1, Heparanase, HPA, HSE1, HEP, HPA1, HPSE1, HPR1
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
Heparan sulfate proteoglycans are major components of the basement membrane and extracellular matrix. The protein encoded by this gene is an enzyme that cleaves heparan sulfate proteoglycans to permit cell movement through remodeling of the extracellular matrix. In addition, this cleavage can release bioactive molecules from the extracellular matrix. Several transcript variants encoding different isoforms have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant protein of human Heparanase 1
NCBI Gene ID #:
10855
NCBI Official Name:
heparanase
NCBI Official Symbol:
HPSE
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 50kDa/65kDa
Purification:
Affinity purification
Research Area:
Cancer, Cell Cycle, Signal Transduction
Swissprot #:
Q9Y251
User NOte:
Optimal dilutions for each application to be determined by the researcher.