PCF11 Antibody

ProSci
Product Code: PSI-16-646
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-16-646-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using PCF11 antibody (16-646) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.

Western blot analysis of extracts of various cell lines, using PCF11 antibody (16-646) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.

Further Information

Additional Names:
pre-mRNA cleavage complex 2 protein Pcf11, PCF11, cleavage and polyadenylation factor subunit, homolog, PCF11p homolog, pre-mRNA cleavage complex II protein Pcf11
Application Note:
WB: 1:500 - 1:2000
Background:
The protein encoded by this gene binds to CLP1 to form pre-mRNA cleavage factor IIm. The encoded protein is necessary for efficient Pol II transcription termination and may be involved in degradation of the 3' product of polyA site cleavage.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1256-1555 of human PCF11 (NP_056969.2).
NCBI Gene ID #:
51585
NCBI Official Name:
PCF11 cleavage and polyadenylation factor subunit
NCBI Official Symbol:
PCF11
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 180kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
O94913
User NOte:
Optimal dilutions for each application to be determined by the researcher.