PPM1K Antibody

ProSci
Product Code: PSI-16-674
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-16-674-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using PPM1K antibody (16-674) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.

Western blot analysis of extracts of various cell lines, using PPM1K antibody (16-674) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.

Further Information

Additional Names:
BDP, MSUDMV, PP2Ckappa, PP2Cm, PTMP, UG0882E07, protein phosphatase 1K, mitochondrial, PP2C domain-containing protein phosphatase 1K, PP2C-kappa, PP2C-type mitochondrial phosphoprotein phosphatase, branched-chain I+/--ketoacid dehydrogenase phosphatase, branched-chain alpha-ketoacid dehydrogenase phosphatase, protein phosphatase 2C kappa
Application Note:
WB: 1:500 - 1:2000
Background:
This gene encodes a member of the PPM family of Mn2+/Mg2+-dependent protein phosphatases. The encoded protein, essential for cell survival and development, is targeted to the mitochondria where it plays a key role in regulation of the mitochondrial permeability transition pore.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 183-372 of human PPM1K (NP_689755.3).
NCBI Gene ID #:
152926
NCBI Official Name:
protein phosphatase, Mg2+/Mn2+ dependent 1K
NCBI Official Symbol:
PPM1K
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 41kDa
Purification:
Affinity purification
Research Area:
Signal Transduction
Swissprot #:
Q8N3J5
User NOte:
Optimal dilutions for each application to be determined by the researcher.