UGT1A9 Antibody

ProSci
Product Code: PSI-16-677
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-16-677-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using UGT1A9 antibody (16-677) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
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Immunohistochemistry of paraffin-embedded human liver cancer using UGT1A9 antibody (16-677) at dilution of 1:200 (40x lens).
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Immunofluorescence analysis of MCF-7 cells using UGT1A9 antibody (16-677). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using UGT1A9 antibody (16-677) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded human liver cancer using UGT1A9 antibody (16-677) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of MCF-7 cells using UGT1A9 antibody (16-677). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
HLUGP4, LUGP4, UDPGT, UDPGT 1-9, UGT-1I, UGT1-09, UGT1-9, UGT1.9S, UGT1AI, UGT1I, UGT1A9, UDP-glucuronosyltransferase 1-9, UDP glucuronosyltransferase 1 family, polypeptide A9, UDP glycosyltransferase 1 family, polypeptide A9, UDP-glucuronosyltransferase 1 family polypeptide A9s, UDP-glucuronosyltransferase 1-I, UDP-glucuronosyltransferase 1A9
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
This gene encodes a UDP-glucuronosyltransferase, an enzyme of the glucuronidation pathway that transforms small lipophilic molecules, such as steroids, bilirubin, hormones, and drugs, into water-soluble, excretable metabolites. This gene is part of a complex locus that encodes several UDP-glucuronosyltransferases. The locus includes thirteen unique alternate first exons followed by four common exons. Four of the alternate first exons are considered pseudogenes. Each of the remaining nine 5' exons may be spliced to the four common exons, resulting in nine proteins with different N-termini and identical C-termini. Each first exon encodes the substrate binding site, and is regulated by its own promoter. The enzyme encoded by this gene is active on phenols.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 26-250 of human UGT1A9 (NP_066307.1).
NCBI Gene ID #:
54600
NCBI Official Name:
UDP glucuronosyltransferase family 1 member A9
NCBI Official Symbol:
UGT1A9
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 71kDa
Purification:
Affinity purification
Research Area:
Cancer, Signal Transduction
Swissprot #:
O60656
User NOte:
Optimal dilutions for each application to be determined by the researcher.