c-Fos Antibody

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ProSci
Product Code: PSI-16-693
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-16-693-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

1 / 3
Western blot analysis of extracts of various cell lines, using c-Fos antibody (16-693) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 1s.
2 / 3
Immunofluorescence analysis of C6 cells using c-Fos antibody (16-693) at dilution of 1:100. Blue: DAPI for nuclear staining.
3 / 3
Immunofluorescence analysis of NIH/3T3 cells using c-Fos antibody (16-693) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using c-Fos antibody (16-693) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 1s.
Immunofluorescence analysis of C6 cells using c-Fos antibody (16-693) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using c-Fos antibody (16-693) at dilution of 1:100. Blue: DAPI for nuclear staining.

Documents

Further Information

Additional Names:
FOS, p55, AP-1, C-FOS
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:200
Background:
The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. In some cases, expression of the FOS gene has also been associated with apoptotic cell death.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant protein of human c-Fos
NCBI Gene ID #:
2353
NCBI Official Name:
FBJ murine osteosarcoma viral oncogene homolog
NCBI Official Symbol:
FOS
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 60kDa
Purification:
Affinity purification
Research Area:
Cancer, Immunology, Neuroscience, Signal Transduction, Transcription
Swissprot #:
P01100
User NOte:
Optimal dilutions for each application to be determined by the researcher.