ERK1 / ERK2 Antibody

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ProSci
Product Code: PSI-16-712
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-16-712-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using ERK1 / ERK2 antibody (16-712) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
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Immunofluorescence analysis of C6 cells using ERK1 / ERK2 antibody (16-712) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of NIH/3T3 cells using ERK1 / ERK2 antibody (16-712) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of U2OS cells using ERK1 / ERK2 antibody (16-712) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of C6 cells using ERK1 / ERK2 Polyclonal Antibody (16-712) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of NIH-3T3 cells using ERK1 / ERK2 Polyclonal Antibody (16-712) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of U-2 OS cells using ERK1 / ERK2 Polyclonal Antibody (16-712) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using ERK1 / ERK2 antibody (16-712) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
Immunofluorescence analysis of C6 cells using ERK1 / ERK2 antibody (16-712) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using ERK1 / ERK2 antibody (16-712) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U2OS cells using ERK1 / ERK2 antibody (16-712) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of C6 cells using ERK1 / ERK2 Polyclonal Antibody (16-712) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH-3T3 cells using ERK1 / ERK2 Polyclonal Antibody (16-712) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using ERK1 / ERK2 Polyclonal Antibody (16-712) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Documents

Further Information

Additional Names:
ERK, ERT1, MAPK 1, MAPK2, ERK2
Application Note:
WB: 1:1000 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant Protein of human ERK1 / ERK2
NCBI Gene ID #:
5594/5595
NCBI Official Name:
mitogen-activated protein kinase 1, mitogen-activated protein kinase 3
NCBI Official Symbol:
MAPK1/MAPK3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 42kDa/44kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
P28482/P27361
User NOte:
Optimal dilutions for each application to be determined by the researcher.