XRCC4 Antibody

ProSci
Product Code: PSI-16-747
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-16-747-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using XRCC4 antibody (16-747) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
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Immunohistochemistry of paraffin-embedded human appendix using XRCC4 antibody (16-747) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human breast cancer using XRCC4 antibody (16-747) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human gastric cancer using XRCC4 antibody (16-747) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of 293T cells using XRCC4 antibody (16-747) at dilution of 1:100. Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using XRCC4 antibody (16-747) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunohistochemistry of paraffin-embedded human appendix using XRCC4 antibody (16-747) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast cancer using XRCC4 antibody (16-747) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using XRCC4 antibody (16-747) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of 293T cells using XRCC4 antibody (16-747) at dilution of 1:100. Blue: DAPI for nuclear staining.

Further Information

Additional Names:
DNA repair protein XRCC4, X-ray repair cross-complementing protein 4, XRCC4
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:10 - 1:100
Background:
The protein encoded by this gene functions together with DNA ligase IV and the DNA-dependent protein kinase in the repair of DNA double-strand breaks. This protein plays a role in both non-homologous end joining and the completion of V(D)J recombination. Mutations in this gene can cause short stature, microcephaly, and endocrine dysfunction (SSMED). Alternative splicing generates several transcript variants.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-336 of human XRCC4 (NP_071801.1).
NCBI Gene ID #:
7518
NCBI Official Name:
DNA repair protein XRCC4
NCBI Official Symbol:
XRCC4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 55kDa
Purification:
Affinity purification
Research Area:
Apoptosis, Cell Cycle
Swissprot #:
Q13426
User NOte:
Optimal dilutions for each application to be determined by the researcher.