PIK3R3 Antibody

ProSci
Product Code: PSI-16-869
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-16-869-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

1 / 2
Western blot analysis of extracts of Mouse brain, using PIK3R3 antibody (16-869) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
2 / 2
Immunofluorescence analysis of NIH-3T3 cells using PIK3R3 antibody (16-869) at dilution of 1:100. Blue: DAPI for nuclear staining.

Western blot analysis of extracts of Mouse brain, using PIK3R3 antibody (16-869) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunofluorescence analysis of NIH-3T3 cells using PIK3R3 antibody (16-869) at dilution of 1:100. Blue: DAPI for nuclear staining.

Further Information

Additional Names:
PIK3R3, DKFZp686P05226, FLJ41892, p55-GAMMA, p55
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100

IF: 1:50 - 1:100
Background:
Phosphatidylinositol 3-kinase (PI3K) phosphorylates phosphatidylinositol and similar compounds, which then serve as second messengers in growth signaling pathways. PI3K is composed of a catalytic and a regulatory subunit. The protein encoded by this gene represents a regulatory subunit of PI3K. The encoded protein contains two SH2 domains through which it binds activated protein tyrosine kinases to regulate their activity.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant protein of human PIK3R3.
NCBI Gene ID #:
8503
NCBI Official Name:
phosphoinositide-3-kinase, regulatory subunit 3 (gamma)
NCBI Official Symbol:
PIK3R3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 54kDa
Purification:
Affinity purification
Research Area:
Apoptosis, Cell Cycle, Immunology, Signal Transduction
Swissprot #:
Q92569
User NOte:
Optimal dilutions for each application to be determined by the researcher.