Lamin A / C Antibody

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ProSci
Product Code: PSI-16-912
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-16-912-50uL50uL£440.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using Lamin A / C antibody (16-912) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 3s.
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Immunohistochemistry of paraffin-embedded human mammary cancer using Lamin A / C antibody (16-912) at dilution of 1:200 (40x lens).
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Immunohistochemistry of paraffin-embedded human uterine cancer using Lamin A / C antibody (16-912) at dilution of 1:200 (40x lens).
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Immunohistochemistry of paraffin-embedded human colon using Lamin A / C antibody (16-912) at dilution of 1:200 (40x lens).
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Immunohistochemistry of paraffin-embedded human stomach using Lamin A / C antibody (16-912) at dilution of 1:200 (40x lens).
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Immunohistochemistry of paraffin-embedded human placenta using Lamin A / C antibody (16-912) at dilution of 1:200 (40x lens).
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Immunohistochemistry of paraffin-embedded rat brain using Lamin A / C antibody (16-912) at dilution of 1:200 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse spinal cord using Lamin A / C antibody (16-912) at dilution of 1:200 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse brain using Lamin A / C antibody (16-912) at dilution of 1:200 (40x lens).
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Immunofluorescence analysis of NIH-3T3 cells using BLOC1S3 antibody (16-904) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of NIH-3T3 cells using Lamin A / C antibody (16-912) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of U-2 OS cells using Lamin A / C antibody (16-912) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using Lamin A / C antibody (16-912) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 3s.
Immunohistochemistry of paraffin-embedded human mammary cancer using Lamin A / C antibody (16-912) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human uterine cancer using Lamin A / C antibody (16-912) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human colon using Lamin A / C antibody (16-912) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using Lamin A / C antibody (16-912) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human placenta using Lamin A / C antibody (16-912) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using Lamin A / C antibody (16-912) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spinal cord using Lamin A / C antibody (16-912) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using Lamin A / C antibody (16-912) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of NIH-3T3 cells using BLOC1S3 antibody (16-904) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH-3T3 cells using Lamin A / C antibody (16-912) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using Lamin A / C antibody (16-912) at dilution of 1:100. Blue: DAPI for nuclear staining.

Documents

Further Information

Additional Names:
LMNA, 70 kDa lamin, CDCD1, CMD1A, EMD2, FPLD, IDC, FPL, FPLD2, Lamin A/C, Lamin, LFP, LMNC, LMN1, LMNL1, HGPS, Prelamin-A/C, PRO1, Lamin A, Lamin A/C-like 1, LGMD1B, CDDC, CMT2B1, LDP1
Application Note:
WB: 1:200 - 1:1000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A synthetic peptide of human Lamin A / C
NCBI Gene ID #:
4000
NCBI Official Name:
lamin A/C
NCBI Official Symbol:
LMNA
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 74kDa
Purification:
Affinity purification
Research Area:
Apoptosis, Cell Cycle, Signal Transduction, Stem Cell
Swissprot #:
P02545
User NOte:
Optimal dilutions for each application to be determined by the researcher.