B4GALT3 Antibody

ProSci
Product Code: PSI-16-988
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-16-988-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using B4GALT3 antibody (16-988) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.

Western blot analysis of extracts of various cell lines, using B4GALT3 antibody (16-988) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.

Further Information

Additional Names:
beta4Gal-T3, beta-1,4-galactosyltransferase 3, N-acetyllactosamine synthase, UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 3, UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 3, UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 3, b4Gal-T3, beta-1,4-GalTase 3, beta-N-acetylglucosaminyl-glycolipid beta-1,4-galactosyltransferase 3, beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase, epididymis secretory sperm binding protein, nal synthase
Application Note:
WB: 1:500 - 1:2000

IHC: 1:100 - 1:200
Background:
This gene is one of seven beta-1,4-galactosyltransferase (beta4GalT) genes. They encode type II membrane-bound glycoproteins that appear to have exclusive specificity for the donor substrate UDP-galactose; all transfer galactose in a beta1,4 linkage to similar acceptor sugars: GlcNAc, Glc, and Xyl. Each beta4GalT has a distinct function in the biosynthesis of different glycoconjugates and saccharide structures. As type II membrane proteins, they have an N-terminal hydrophobic signal sequence that directs the protein to the Golgi apparatus and which then remains uncleaved to function as a transmembrane anchor. By sequence similarity, the beta4GalTs form four groups: beta4GalT1 and beta4GalT2, beta4GalT3 and beta4GalT4, beta4GalT5 and beta4GalT6, and beta4GalT7. This gene encodes an enzyme that may be mainly involved in the synthesis of the first N-acetyllactosamine unit of poly-N-acetyllactosamine chains. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Dec 2010]
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 40-150 of human B4GALT3 (NP_003770.1).
NCBI Gene ID #:
8703
NCBI Official Name:
beta-1,4-galactosyltransferase 3
NCBI Official Symbol:
B4GALT3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 44kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
O60512
User NOte:
Optimal dilutions for each application to be determined by the researcher.