DHX29 Antibody

ProSci
Product Code: PSI-18-059
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-18-059-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of HepG2 cells, using DHX29 antibody (18-059) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.

Western blot analysis of extracts of HepG2 cells, using DHX29 antibody (18-059) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.

Further Information

Additional Names:
DDX29, ATP-dependent RNA helicase DHX29, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 29, DEAH (Asp-Glu-Ala-His) box polypeptide 29, DEAH box protein 29, DEAH-box helicase 29, nucleic acid helicase DDXx
Application Note:
WB: 1:500 - 1:2000
Background:
This gene encodes a member of the DEAH (Asp-Glu-Ala-His) subfamily of proteins, part of the DEAD (Asp-Glu-Ala-Asp) box family of RNA helicases. The encoded protein functions in translation initiation, and is specifically required for ribosomal scanning across stable mRNA secondary structures during initiation codon selection. This protein may also play a role in sensing virally derived cytosolic nucleic acids. Knockdown of this gene results in reduced protein translation and impaired proliferation of cancer cells. [provided by RefSeq, Sep 2016]
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1020-1369 of human DHX29 (NP_061903.2).
NCBI Gene ID #:
54505
NCBI Official Name:
DExH-box helicase 29
NCBI Official Symbol:
DHX29
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 155kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
Q7Z478
User NOte:
Optimal dilutions for each application to be determined by the researcher.