Cyclin D2 Antibody

ProSci
Product Code: PSI-18-075
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-18-075-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using Cyclin D2 antibody (18-075) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
2 / 4
Immunohistochemistry of paraffin-embedded human stomach using Cyclin D2 antibody (18-075) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse brain using Cyclin D2 antibody (18-075) at dilution of 1:100 (40x lens).
4 / 4
Immunofluorescence analysis of U2OS cells using Cyclin D2 antibody (18-075) at dilution of 1:100. Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using Cyclin D2 antibody (18-075) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
Immunohistochemistry of paraffin-embedded human stomach using Cyclin D2 antibody (18-075) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using Cyclin D2 antibody (18-075) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using Cyclin D2 antibody (18-075) at dilution of 1:100. Blue: DAPI for nuclear staining.

Further Information

Additional Names:
KIAK0002, MPPH3, G1/S-specific cyclin-D2
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100

IF: 1:50 - 1:100
Background:
The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with CDK4 or CDK6 and functions as a regulatory subunit of the complex, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with and be involved in the phosphorylation of tumor suppressor protein Rb. Knockout studies of the homologous gene in mouse suggest the essential roles of this gene in ovarian granulosa and germ cell proliferation. High level expression of this gene was observed in ovarian and testicular tumors. Mutations in this gene are associated with megalencephaly-polymicrogyria-polydactyly-hydrocephalus syndrome 3 (MPPH3).
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-289 of human Cyclin D2 (NP_001750.1).
NCBI Gene ID #:
894
NCBI Official Name:
cyclin D2
NCBI Official Symbol:
CCND2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 37kDa
Purification:
Affinity purification
Research Area:
Cancer, Cell Cycle
Swissprot #:
P30279
User NOte:
Optimal dilutions for each application to be determined by the researcher.