MIB2 Antibody

ProSci
Product Code: PSI-18-124
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-18-124-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using MIB2 antibody (18-124) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 60s.

Western blot analysis of extracts of various cell lines, using MIB2 antibody (18-124) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 60s.

Further Information

Additional Names:
ZZANK1, ZZZ5, E3 ubiquitin-protein ligase MIB2, RING-type E3 ubiquitin transferase MIB2, mind bomb homolog 2, mindbomb homolog 2, novel zinc finger protein, novelzin, putative NF-kappa-B-activating protein 002N, skeletrophin, zinc finger ZZ type with ankyrin repeat domain protein 1, zinc finger, ZZ type with ankyrin repeat domain 1
Application Note:
WB: 1:500 - 1:2000
Background:
The protein encoded by this gene is an E3 ubiquitin protein ligase that mediates ubiquitination of proteins in the Notch signaling pathway. The encoded protein may be a suppressor of melanoma invasion. [provided by RefSeq, Mar 2017]
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 300-580 of human MIB2 (NP_001164158.1).
NCBI Gene ID #:
142678
NCBI Official Name:
mindbomb E3 ubiquitin protein ligase 2
NCBI Official Symbol:
MIB2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 110kDa
Purification:
Affinity purification
Research Area:
Cell Cycle
Swissprot #:
Q96AX9
User NOte:
Optimal dilutions for each application to be determined by the researcher.