EEF1A1 Antibody

ProSci
Product Code: PSI-18-137
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-18-137-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using EEF1A1 antibody (18-137) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
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Immunohistochemistry of paraffin-embedded human colon carcinoma using EEF1A1 antibody (18-137) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human liver cancer using EEF1A1 antibody (18-137) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded rat heart using EEF1A1 antibody (18-137) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of C6 cells using EEF1A1 antibody (18-137) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of NIH/3T3 cells using EEF1A1 antibody (18-137) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of U2OS cells using EEF1A1 antibody (18-137) at dilution of 1:100. Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using EEF1A1 antibody (18-137) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
Immunohistochemistry of paraffin-embedded human colon carcinoma using EEF1A1 antibody (18-137) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using EEF1A1 antibody (18-137) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using EEF1A1 antibody (18-137) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of C6 cells using EEF1A1 antibody (18-137) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using EEF1A1 antibody (18-137) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U2OS cells using EEF1A1 antibody (18-137) at dilution of 1:100. Blue: DAPI for nuclear staining.

Further Information

Additional Names:
EEF1A1, CCS-3, CCS3, EEF-1, EEF1A, EF-Tu, EF1A, FLJ25721, GRAF-1EF, HNGC:16303, LENG7, MGC102687, MGC131894, MGC16224, PTI1, eEF1A-1, EE1A1
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
This gene encodes an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas, and the other isoform (alpha 2) is expressed in brain, heart and skeletal muscle. This isoform is identified as an autoantigen in 66% of patients with Felty syndrome. This gene has been found to have multiple copies on many chromosomes, some of which, if not all, represent different pseudogenes.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 123-462 of human EEF1A1 (NP_001393.1).
NCBI Gene ID #:
1915
NCBI Official Name:
eukaryotic translation elongation factor 1 alpha 1
NCBI Official Symbol:
EEF1A1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
P68104
User NOte:
Optimal dilutions for each application to be determined by the researcher.