TFF1 Antibody

ProSci
Product Code: PSI-18-153
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-18-153-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of BT-474 cells, using TFF1 antibody (18-153) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded human breast cancer using TFF1 antibody (18-153) at dilution of 1:200 (40x lens).
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Immunohistochemistry of paraffin-embedded human liver cancer using TFF1 antibody (18-153) at dilution of 1:200 (40x lens).
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Immunohistochemistry of paraffin-embedded rat lung using TFF1 antibody (18-153) at dilution of 1:200 (40x lens).
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Immunohistochemistry of paraffin-embedded rat kidney using TFF1 antibody (18-153) at dilution of 1:200 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse brain using TFF1 antibody (18-153) at dilution of 1:200 (40x lens).
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Immunohistochemistry of paraffin-embedded human colon carcinoma using TFF1 antibody (18-153) at dilution of 1:200 (40x lens).
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Immunohistochemistry of paraffin-embedded human colon carcinoma using TFF1 antibody (18-153) at dilution of 1:200 (40x lens).
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Immunohistochemistry of paraffin-embedded human stomach using TFF1 antibody (18-153) at dilution of 1:200 (40x lens).

Western blot analysis of extracts of BT-474 cells, using TFF1 antibody (18-153) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human breast cancer using TFF1 antibody (18-153) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using TFF1 antibody (18-153) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat lung using TFF1 antibody (18-153) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using TFF1 antibody (18-153) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using TFF1 antibody (18-153) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using TFF1 antibody (18-153) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using TFF1 antibody (18-153) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using TFF1 antibody (18-153) at dilution of 1:200 (40x lens).

Further Information

Additional Names:
Trefoil factor 1, Breast cancer estrogen-inducible protein, PNR-2, Polypeptide P1.A, hP1.A, Protein pS2, TFF1, BCEI, PS2
Application Note:
WB: 1:500 - 1:1000

IHC: 1:50 - 1:200
Background:
Members of the trefoil family are characterized by having at least one copy of the trefoil motif, a 40-amino acid domain that contains three conserved disulfides. They are stable secretory proteins expressed in gastrointestinal mucosa. Their functions are not defined, but they may protect the mucosa from insults, stabilize the mucus layer, and affect healing of the epithelium. This gene, which is expressed in the gastric mucosa, has also been studied because of its expression in human tumors. This gene and two other related trefoil family member genes are found in a cluster on chromosome 21.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 25-84 of human TFF1 (NP_003216.1).
NCBI Gene ID #:
7031
NCBI Official Name:
trefoil factor 1
NCBI Official Symbol:
TFF1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 12kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Growth Factors, Signal Transduction
Swissprot #:
P04155
User NOte:
Optimal dilutions for each application to be determined by the researcher.