XRCC2 Antibody

ProSci
Product Code: PSI-18-186
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-18-186-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

1 / 4
Western blot analysis of extracts of various cell lines, using XRCC2 antibody (18-186) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
2 / 4
Immunohistochemistry of paraffin-embedded human thyroid cancer using XRCC2 antibody (18-186) at dilution of 1:200 (40x lens).
3 / 4
Immunofluorescence analysis of MCF-7 cells using XRCC2 antibody (18-186). Blue: DAPI for nuclear staining.
4 / 4
Immunofluorescence analysis of A549 cells using XRCC2 antibody (18-186).

Western blot analysis of extracts of various cell lines, using XRCC2 antibody (18-186) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunohistochemistry of paraffin-embedded human thyroid cancer using XRCC2 antibody (18-186) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of MCF-7 cells using XRCC2 antibody (18-186). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of A549 cells using XRCC2 antibody (18-186).

Further Information

Additional Names:
FANCU, DNA repair protein XRCC2, X-ray repair complementing defective repair in Chinese hamster cells 2, X-ray repair cross-complementing protein 2, epididymis secretory sperm binding protein
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
This gene encodes a member of the RecA/Rad51-related protein family that participates in homologous recombination to maintain chromosome stability and repair DNA damage. This gene is involved in the repair of DNA double-strand breaks by homologous recombination and it functionally complements Chinese hamster irs1, a repair-deficient mutant that exhibits hypersensitivity to a number of different DNA-damaging agents.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human XRCC2 (NP_005422.1).
NCBI Gene ID #:
7516
NCBI Official Name:
X-ray repair cross complementing 2
NCBI Official Symbol:
XRCC2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 37kDa
Purification:
Affinity purification
Research Area:
Apoptosis, Cell Cycle
Swissprot #:
O43543
User NOte:
Optimal dilutions for each application to be determined by the researcher.