AKT2 Antibody [8G3B6], KO Validated

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ProSci
Product Code: PSI-18-206
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-18-206-50uL50uL£505.00
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Overview

Host Type: Mouse
Antibody Isotype: IgG
Antibody Clonality: Monoclonal
Antibody Clone: [8G3B6]
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts from normal (control) and AKT2 knockout (KO) 293T cells, using AKT2 antibody (18-206) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
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Western blot analysis of extracts from normal (control) and AKT2 knockout (KO) 293T cells, using AKT2 antibody (18-206) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.

Documents

Further Information

Additional Names:
rac protein kinase beta, Murine thymoma viral (v-akt) homolog-2, RAC-BETA, PRKBB, PKBBETA, HGNC:392, v-akt murine thymoma viral oncogene homolog 2, AKT2, PKBB, HIHGHH
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100

ICC: 1:50 - 1:200

IF: 1:50 - 1:100

IP: 1:20 - 1:50

ChIP: 1:20 - 1:100
Background:
This gene is a putative oncogene encoding a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains. The gene was shown to be amplified and overexpressed in 2 of 8 ovarian carcinoma cell lines and 2 of 15 primary ovarian tumors. Overexpression contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. The encoded protein is a general protein kinase capable of phophorylating several known proteins.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant protein of human AKT2
NCBI Gene ID #:
208
NCBI Official Name:
v-akt murine thymoma viral oncogene homolog 2
NCBI Official Symbol:
AKT2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 60kDa
Purification:
Affinity purification
Research Area:
Apoptosis, Cancer, Cell Cycle, Immunology, Neuroscience, Signal Transduction
Swissprot #:
P31751
User NOte:
Optimal dilutions for each application to be determined by the researcher.
VALIDATION:
Antibody is Knockout validated.