ANXA6 Antibody, KO Validated

ProSci
Product Code: PSI-18-259
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-18-259-50uL50uL£505.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts from normal (control) and ANXA6 knockout (KO) HeLa cells, using ANXA6 antibody (18-259) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.

Western blot analysis of extracts from normal (control) and ANXA6 knockout (KO) HeLa cells, using ANXA6 antibody (18-259) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.

Further Information

Additional Names:
ANXA6, ANX6, CBP68
Application Note:
WB: 1:500 - 1:2000
Background:
Annexin VI belongs to a family of calcium-dependent membrane and phospholipid binding proteins. Several members of the annexin family have been implicated in membrane-related events along exocytotic and endocytotic pathways. The annexin VI gene is approximately 60 kbp long and contains 26 exons. It encodes a protein of about 68 kDa that consists of eight 68-amino acid repeats separated by linking sequences of variable lengths. It is highly similar to human annexins I and II sequences, each of which contain four such repeats. Annexin VI has been implicated in mediating the endosome aggregation and vesicle fusion in secreting epithelia during exocytosis. Alternatively spliced transcript variants have been described.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human ANXA6 (NP_001146.2).
NCBI Gene ID #:
309
NCBI Official Name:
annexin A6
NCBI Official Symbol:
ANXA6
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 76kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Neuroscience, Signal Transduction
Swissprot #:
P08133
User NOte:
Optimal dilutions for each application to be determined by the researcher.
VALIDATION:
Antibody is Knockout validated.