ALDH9A1 Antibody, KO Validated

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ProSci
Product Code: PSI-18-289
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-18-289-50uL50uL£505.00
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Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts from normal (control) and ALDH9A1 knockout (KO) 293T cells, using ALDH9A1 antibody (18-289) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.
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Western blot analysis of extracts from normal (control) and ALDH9A1 knockout (KO) 293T cells, using ALDH9A1 antibody (18-289) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.

Documents

Further Information

Additional Names:
ALDH9A1, TMABADH, R-aminobutyraldehyde dehydrogenase, E3, ALDH9, ALDH7, ALDH4, aldehyde dehydrogenase E3 is, aldehyde dehydrogenase 9A1, aldehyde dehydrogenase 9 family, member A1, aldehyde dehydrogenase (NAD+), 4-trimethylaminobutyraldehyde dehydrogenase
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100
Background:
This protein belongs to the aldehyde dehydrogenase family of proteins. It has a high activity for oxidation of gamma-aminobutyraldehyde and other amino aldehydes. The enzyme catalyzes the dehydrogenation of gamma-aminobutyraldehyde to gamma-aminobutyric acid (GABA). This isozyme is a tetramer of identical 54-kD subunits.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A synthetic peptide of human ALDH9A1
NCBI Gene ID #:
223
NCBI Official Name:
aldehyde dehydrogenase 9 family, member A1
NCBI Official Symbol:
ALDH9A1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 54kDa
Purification:
Affinity purification
Research Area:
Cancer, Neuroscience, Signal Transduction
Swissprot #:
P49189
User NOte:
Optimal dilutions for each application to be determined by the researcher.
VALIDATION:
Antibody is Knockout validated.