PIP5K1A Antibody, KO Validated

ProSci
Product Code: PSI-18-290
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-18-290-50uL50uL£505.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts from normal (control) and PIP5K1A knockout (KO) 293T cells, using PIP5K1A antibody (18-290) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.

Western blot analysis of extracts from normal (control) and PIP5K1A knockout (KO) 293T cells, using PIP5K1A antibody (18-290) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.

Further Information

Additional Names:
Phosphatidylinositol 4-phosphate 5-kinase type-1 alpha, PIP5K1-alpha, PtdIns(4)P-5-kinase 1 alpha, 68 kDa type I phosphatidylinositol 4-phosphate 5-kinase alpha, Phosphatidylinositol 4-phosphate 5-kinase type I alpha, PIP5KIalpha, PIP5K1A
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:100
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 231-500 of human PIP5K1A (NP_001129109.1).
NCBI Gene ID #:
8394
NCBI Official Name:
Phosphatidylinositol 4-phosphate 5-kinase type-1 alpha
NCBI Official Symbol:
PIP5K1A
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 63kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Signal Transduction
Swissprot #:
Q99755
User NOte:
Optimal dilutions for each application to be determined by the researcher.
VALIDATION:
Antibody is Knockout validated.