HSD3B2 Antibody

ProSci
Product Code: PSI-18-323
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-18-323-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using HSD3B2 antibody (18-323) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
2 / 2
Immunofluorescence analysis of A-549 cells using HSD3B2 antibody (18-323).

Western blot analysis of extracts of various cell lines, using HSD3B2 antibody (18-323) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunofluorescence analysis of A-549 cells using HSD3B2 antibody (18-323).

Further Information

Additional Names:
HSD3B2, HSDB, HSDB3, HSD3B, SDR11E2
Application Note:
WB: 1:500 - 1:2000

IF: 1:10 - 1:100
Background:
The protein encoded by this gene is a bifunctional enzyme that catalyzes the oxidative conversion of delta(5)-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. It plays a crucial role in the biosynthesis of all classes of hormonal steroids. This gene is predominantly expressed in the adrenals and the gonads. Mutations in this gene are associated with 3-beta-hydroxysteroid dehydrogenase, type II, deficiency. Alternatively spliced transcript variants have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-287 of human HSD3B2 (NP_000189.1).
NCBI Gene ID #:
3284
NCBI Official Name:
hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 2
NCBI Official Symbol:
HSD3B2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 47kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
P26439
User NOte:
Optimal dilutions for each application to be determined by the researcher.