POLR2F Antibody

ProSci
Product Code: PSI-18-328
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-18-328-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of SW620 cells, using POLR2F antibody (18-328) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
2 / 2
Immunofluorescence analysis of HeLa cells using POLR2F antibody (18-328). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of SW620 cells, using POLR2F antibody (18-328) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunofluorescence analysis of HeLa cells using POLR2F antibody (18-328). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
HRBP14.4, POLRF, RPABC14.4, RPABC2, RPB14.4, RPB6, RPC15, DNA-directed RNA polymerases I, II, and III subunit RPABC2, DNA-directed RNA polymerase II subunit F, DNA-directed RNA polymerases I, II, and III 14.4 kDa polypeptide, RNA Polymerase II subunit 14.4 kD, RNA polymerases I, II, and III subunit ABC2, polymerase (RNA) II (DNA directed) polypeptide F, polymerase (RNA) II subunit F
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:100
Background:
This gene encodes the sixth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. In yeast, this polymerase subunit, in combination with at least two other subunits, forms a structure that stabilizes the transcribing polymerase on the DNA template. Alternative splicing results in multiple transcript variants.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-127 of human POLR2F (NP_068809.1).
NCBI Gene ID #:
5435
NCBI Official Name:
RNA polymerase II subunit F
NCBI Official Symbol:
POLR2F
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 14kDa
Purification:
Affinity purification
Research Area:
Neuroscience
Swissprot #:
P61218
User NOte:
Optimal dilutions for each application to be determined by the researcher.