GRIA1 Antibody

ProSci
Product Code: PSI-18-331
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-18-331-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of BT-474 cells, using GRIA1 antibody (18-331) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
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Immunofluorescence analysis of rat brain using GRIA1 antibody (18-331) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of mouse brain using GRIA1 antibody (18-331) at dilution of 1:100. Blue: DAPI for nuclear staining.

Western blot analysis of extracts of BT-474 cells, using GRIA1 antibody (18-331) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunofluorescence analysis of rat brain using GRIA1 antibody (18-331) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of mouse brain using GRIA1 antibody (18-331) at dilution of 1:100. Blue: DAPI for nuclear staining.

Further Information

Additional Names:
glutamate receptor 1, gluR-K1, gluR-A, gluR-1, AMPA 1, MGC133252, HBGR1, GluA1, GLURA, GLUR1, GLUH1, glutamate receptor, ionotropic, GRIA1
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:200
Background:
Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. These receptors are heteromeric protein complexes with multiple subunits, each possessing transmembrane regions, and all arranged to form a ligand-gated ion channel. The classification of glutamate receptors is based on their activation by different pharmacologic agonists. This gene belongs to a family of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA) receptors. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 19-300 of human GRIA1 (NP_000818.2).
NCBI Gene ID #:
2890
NCBI Official Name:
glutamate receptor, ionotropic, AMPA 1
NCBI Official Symbol:
GRIA1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 110kDa
Purification:
Affinity purification
Research Area:
Neuroscience
Swissprot #:
P42261
User NOte:
Optimal dilutions for each application to be determined by the researcher.