RBX1 Antibody

ProSci
Product Code: PSI-18-336
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-18-336-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using RBX1 antibody (18-336) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
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Immunofluorescence analysis of MCF-7 cells using RBX1 antibody (18-336). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using RBX1 antibody (18-336) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunofluorescence analysis of MCF-7 cells using RBX1 antibody (18-336). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
RBX1, ROC1, RNF75, BA554C12.1
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:200
Background:
This locus encodes a RING finger-like domain-containing protein. The encoded protein interacts with cullin proteins and likely plays a role in ubiquitination processes necessary for cell cycle progression. This protein may also affect protein turnover. Related pseudogenes exist on chromosomes 2 and 5.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-108 of human RBX1 (NP_055063.1).
NCBI Gene ID #:
9978
NCBI Official Name:
ring-box 1, E3 ubiquitin protein ligase
NCBI Official Symbol:
RBX1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 14kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Immunology, Signal Transduction
Swissprot #:
P62877
User NOte:
Optimal dilutions for each application to be determined by the researcher.