PTGIR Antibody

ProSci
Product Code: PSI-18-344
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-18-344-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using PTGIR antibody (18-344) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 90s.
2 / 5
Immunohistochemistry of paraffin-embedded human gastric cancer using PTGIR antibody (18-344) at dilution of 1:200 (40x lens).
3 / 5
Immunohistochemistry of paraffin-embedded human stomach using PTGIR antibody (18-344) at dilution of 1:200 (40x lens).
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Immunohistochemistry of paraffin-embedded human colon carcinoma using PTGIR antibody (18-344) at dilution of 1:200 (40x lens).
5 / 5
Immunohistochemistry of paraffin-embedded human thyroid cancer using PTGIR antibody (18-344) at dilution of 1:200 (40x lens).

Western blot analysis of extracts of various cell lines, using PTGIR antibody (18-344) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human gastric cancer using PTGIR antibody (18-344) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using PTGIR antibody (18-344) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using PTGIR antibody (18-344) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human thyroid cancer using PTGIR antibody (18-344) at dilution of 1:200 (40x lens).

Further Information

Additional Names:
Prostacyclin receptor, Prostaglandin I2 receptor, PGI receptor, PGI2 receptor, Prostanoid IP receptor, PTGIR, PRIPR
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200
Background:
The protein encoded by this gene is a member of the G-protein coupled receptor family 1 and has been shown to be a receptor for prostacyclin. Prostacyclin, the major product of cyclooxygenase in macrovascular endothelium, elicits a potent vasodilation and inhibition of platelet aggregation through binding to this receptor.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 207-386 of human PTGIR (NP_000951.1).
NCBI Gene ID #:
5739
NCBI Official Name:
Prostacyclin receptor
NCBI Official Symbol:
PTGIR
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 41kDa
Purification:
Affinity purification
Research Area:
Signal Transduction
Swissprot #:
P43119
User NOte:
Optimal dilutions for each application to be determined by the researcher.