HSPA1L Antibody

ProSci
Product Code: PSI-18-349
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-18-349-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using HSPA1L antibody (18-349) at 1:500 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
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Immunofluorescence analysis of U2OS cells using HSPA1L antibody (18-349). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using HSPA1L antibody (18-349) at 1:500 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunofluorescence analysis of U2OS cells using HSPA1L antibody (18-349). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
HSPA1L, HSP70-HOM, hum70t, HSP70T, HSP70-1L
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:200
Background:
This gene encodes a 70kDa heat shock protein. In conjunction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which also encode isoforms of the 70kDa heat shock protein.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 342-641 of human HSPA1L (NP_005518.3).
NCBI Gene ID #:
3305
NCBI Official Name:
heat shock 70kDa protein 1-like
NCBI Official Symbol:
HSPA1L
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 67kDa
Purification:
Affinity purification
Research Area:
Signal Transduction
Swissprot #:
P34931
User NOte:
Optimal dilutions for each application to be determined by the researcher.