POLR2D Antibody

ProSci
Product Code: PSI-18-352
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-18-352-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using POLR2D antibody (18-352) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
2 / 3
Immunofluorescence analysis of A549 cells using POLR2D antibody (18-352).
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Immunoprecipitation analysis of 200ug extracts of HepG2 cells using 1ug POLR2D antibody (18-352). Western blot was performed from the immunoprecipitate using POLR2D antibody (18-352) at a dilition of 1:1000.

Western blot analysis of extracts of various cell lines, using POLR2D antibody (18-352) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunofluorescence analysis of A549 cells using POLR2D antibody (18-352).
Immunoprecipitation analysis of 200ug extracts of HepG2 cells using 1ug POLR2D antibody (18-352). Western blot was performed from the immunoprecipitate using POLR2D antibody (18-352) at a dilition of 1:1000.

Further Information

Additional Names:
DNA-directed RNA polymerase II subunit RPB4, RNA polymerase II subunit B4, DNA-directed RNA polymerase II subunit D, RNA polymerase II 16 kDa subunit, RPB16, POLR2D
Application Note:
WB: 1:500 - 1:2000

IF: 1:10 - 1:100

IP: 1:50 - 1:200
Background:
This gene encodes the fourth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. In yeast, this polymerase subunit is associated with the polymerase under suboptimal growth conditions and may have a stress protective role. A sequence for a ribosomal pseudogene is contained within the 3' untranslated region of the transcript from this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-142 of human POLR2D (NP_004796.1).
NCBI Gene ID #:
5433
NCBI Official Name:
DNA-directed RNA polymerase II subunit RPB4
NCBI Official Symbol:
POLR2D
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 17kDa
Purification:
Affinity purification
Research Area:
Neuroscience
Swissprot #:
O15514
User NOte:
Optimal dilutions for each application to be determined by the researcher.