RAD9A Antibody

ProSci
Product Code: PSI-18-367
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-18-367-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of mouse testis, using RAD9A antibody (18-367) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
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Immunohistochemistry of paraffin-embedded rat liver using RAD9A antibody (18-367) at dilution of 1:200 (40x lens).
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Immunohistochemistry of paraffin-embedded human liver damage using RAD9A antibody (18-367) at dilution of 1:200 (40x lens).
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Immunofluorescence analysis of MCF-7 cells using RAD9A antibody (18-367). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of mouse testis, using RAD9A antibody (18-367) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded rat liver using RAD9A antibody (18-367) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human liver damage using RAD9A antibody (18-367) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of MCF-7 cells using RAD9A antibody (18-367). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
RAD9A, RAD9 homolog A (S. pombe), RAD9, RAD9 (S. pombe) homolog, RAD9 homolog, RAD9 homolog (S. pombe), cell cycle checkpoint control protein
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
This gene product is highly similar to Schizosaccharomyces pombe rad9, a cell cycle checkpoint protein required for cell cycle arrest and DNA damage repair. This protein possesses 3' to 5' exonuclease activity, which may contribute to its role in sensing and repairing DNA damage. It forms a checkpoint protein complex with RAD1 and HUS1. This complex is recruited by checkpoint protein RAD17 to the sites of DNA damage, which is thought to be important for triggering the checkpoint-signaling cascade. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 162-391 of human RAD9A (NP_004575.1).
NCBI Gene ID #:
5883
NCBI Official Name:
RAD9 homolog A (S. pombe)
NCBI Official Symbol:
RAD9A
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 27kDa
Purification:
Affinity purification
Research Area:
Apoptosis, Cell Cycle
Swissprot #:
Q99638
User NOte:
Optimal dilutions for each application to be determined by the researcher.