RUVBL2 Antibody

ProSci
Product Code: PSI-18-374
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-18-374-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using RUVBL2 antibody (18-374) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded rat brain using RUVBL2 antibody (18-374) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human lung cancer using RUVBL2 antibody (18-374) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of U2OS cells using RUVBL2 antibody (18-374). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using RUVBL2 antibody (18-374) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat brain using RUVBL2 antibody (18-374) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using RUVBL2 antibody (18-374) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using RUVBL2 antibody (18-374). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
RUVBL2, RVB2, TIH2, ECP51, TIP48, CGI-46, INO80J, REPTIN, TIP49B
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200

IP: 1:50 - 1:100
Background:
This gene encodes the second human homologue of the bacterial RuvB gene. Bacterial RuvB protein is a DNA helicase essential for homologous recombination and DNA double-strand break repair. Functional analysis showed that this gene product has both ATPase and DNA helicase activities. This gene is physically linked to the CGB/LHB gene cluster on chromosome 19q13.3, and is very close (55 nt) to the LHB gene, in the opposite orientation.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-463 of human RUVBL2 (NP_006657.1).
NCBI Gene ID #:
10856
NCBI Official Name:
RuvB-like AAA ATPase 2
NCBI Official Symbol:
RUVBL2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 50kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
Q9Y230
User NOte:
Optimal dilutions for each application to be determined by the researcher.