FHL2 Antibody

ProSci
Product Code: PSI-18-376
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-18-376-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of ES-2 cells, using FHL2 antibody (18-376) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.
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Immunohistochemistry of paraffin-embedded rat kidney using FHL2 antibody (18-376) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse heart using FHL2 antibody (18-376) at dilution of 1:100 (40x lens).

Western blot analysis of extracts of ES-2 cells, using FHL2 antibody (18-376) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.
Immunohistochemistry of paraffin-embedded rat kidney using FHL2 antibody (18-376) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using FHL2 antibody (18-376) at dilution of 1:100 (40x lens).

Further Information

Additional Names:
FHL2, DRAL, AAG11, FHL-2, SLIM3, SLIM-3
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200
Background:
This gene encodes a member of the four-and-a-half-LIM-only protein family. Family members contain two highly conserved, tandemly arranged, zinc finger domains with four highly conserved cysteines binding a zinc atom in each zinc finger. This protein is thought to have a role in the assembly of extracellular membranes. Also, this gene is down-regulated during transformation of normal myoblasts to rhabdomyosarcoma cells and the encoded protein may function as a link between presenilin-2 and an intracellular signaling pathway. Multiple alternatively spliced variants encoding different isoforms have been identified.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-279 of human FHL2 (NP_001034581.1).
NCBI Gene ID #:
2274
NCBI Official Name:
four and a half LIM domains 2
NCBI Official Symbol:
FHL2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 32kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
Q14192
User NOte:
Optimal dilutions for each application to be determined by the researcher.