Lamin B1 Antibody, KO Validated

ProSci
Product Code: PSI-18-377
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-18-377-50uL50uL£505.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using Lamin B1 antibody (18-377) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
2 / 9
Western blot analysis of extracts from normal (control) and Lamin B1 knockout (KO) HeLa cells, using Lamin B1 antibody (18-377) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded rat spinal cord using Lamin B1 antibody (18-377) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded rat heart using Lamin B1 antibody (18-377) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of C6 cells using Lamin B1 antibody (18-377) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of NIH/3T3 cells using Lamin B1 antibody (18-377) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of U2OS cells using Lamin B1 antibody (18-377) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of C6 cells using Lamin B1 Polyclonal Antibody (18-377) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of U-2 OS cells using Lamin B1 Polyclonal Antibody (18-377) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using Lamin B1 antibody (18-377) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Western blot analysis of extracts from normal (control) and Lamin B1 knockout (KO) HeLa cells, using Lamin B1 antibody (18-377) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat spinal cord using Lamin B1 antibody (18-377) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using Lamin B1 antibody (18-377) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of C6 cells using Lamin B1 antibody (18-377) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using Lamin B1 antibody (18-377) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U2OS cells using Lamin B1 antibody (18-377) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of C6 cells using Lamin B1 Polyclonal Antibody (18-377) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using Lamin B1 Polyclonal Antibody (18-377) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
LMNB1, lamin B1, ADLD, LMN, LMN2, LMNB, MGC111419, OTTHUMP00000159218, lamin-B1
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
This gene encodes one of the two B-type lamin proteins and is a component of the nuclear lamina. A duplication of this gene is associated with autosomal dominant adult-onset leukodystrophy (ADLD). Alternative splicing results in multiple transcript variants.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 397-586 of human Lamin B1 (NP_005564.1).
NCBI Gene ID #:
4001
NCBI Official Name:
lamin B1
NCBI Official Symbol:
LMNB1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 70kDa
Purification:
Affinity purification
Research Area:
Apoptosis, Cell Cycle, Signal Transduction
Swissprot #:
P20700
User NOte:
Optimal dilutions for each application to be determined by the researcher.
VALIDATION:
Antibody is Knockout validated.