PRDX2 Antibody, KO Validated

Contact us
ProSci
Product Code: PSI-18-385
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-18-385-50uL50uL£505.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

1 / 3
Western blot analysis of extracts of various cell lines, using PRDX2 antibody (18-385) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
2 / 3
Western blot analysis of extracts from normal (control) and PRDX2 knockout (KO) 293T cells, using PRDX2 antibody (18-385) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.
3 / 3
Immunofluorescence analysis of HeLa cells using PRDX2 antibody (18-385).
❮❯

Western blot analysis of extracts of various cell lines, using PRDX2 antibody (18-385) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Western blot analysis of extracts from normal (control) and PRDX2 knockout (KO) 293T cells, using PRDX2 antibody (18-385) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.
Immunofluorescence analysis of HeLa cells using PRDX2 antibody (18-385).

Documents

Further Information

Additional Names:
PRDX2, peroxiredoxin 2, HGNC:9353, MGC4104, NKEFB, PRP, PRXII, TDPX1, TSA, natural killer-enhancing factor B, thiol-specific antioxidant 1, thioredoxin peroxidase 1, thioredoxin-dependent peroxide reductase 1, torin
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:200
Background:
This gene encodes a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. The encoded protein plays an antioxidant protective role in cells, and it may contribute to the antiviral activity of CD8(+) T-cells. The crystal structure of this protein has been resolved to 2.7 angstroms. This protein prevents hemolytic anemia from oxidative stress by stabilizing hemoglobin, thus making this gene a therapeutic target for patients with hemolytic anemia. This protein may have a proliferative effect and play a role in cancer development or progression. Related pseudogenes have been identified on chromosomes 5, 6, 10 and 13.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-142 of human PRDX2 (NP_859428.1).
NCBI Gene ID #:
7001
NCBI Official Name:
peroxiredoxin 2
NCBI Official Symbol:
PRDX2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 20kDa
Purification:
Affinity purification
Research Area:
Cancer, Cell Cycle, Neuroscience
Swissprot #:
P32119
User NOte:
Optimal dilutions for each application to be determined by the researcher.
VALIDATION:
Antibody is Knockout validated.