DLC1 Antibody

ProSci
Product Code: PSI-18-386
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-18-386-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of mouse lung, using DLC1 antibody (18-386) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.
2 / 2
Immunohistochemistry of paraffin-embedded rat kidney using DLC1 antibody (18-386) at dilution of 1:200 (40x lens).

Western blot analysis of extracts of mouse lung, using DLC1 antibody (18-386) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.
Immunohistochemistry of paraffin-embedded rat kidney using DLC1 antibody (18-386) at dilution of 1:200 (40x lens).

Further Information

Additional Names:
DLC1, ARHGAP7, FLJ21120, HP, STARD12, p122-RhoGAP
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200
Background:
This gene encodes a GTPase-activating protein (GAP) that is a member of the rhoGAP family of proteins which play a role in the regulation of small GTP-binding proteins. GAP family proteins participate in signaling pathways that regulate cell processes involved in cytoskeletal changes. This gene functions as a tumor suppressor gene in a number of common cancers, including prostate, lung, colorectal, and breast cancers. Multiple transcript variants due to alternative promoters and alternative splicing have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 204-463 of human DLC1 (NP_079043.3).
NCBI Gene ID #:
10395
NCBI Official Name:
deleted in liver cancer 1
NCBI Official Symbol:
DLC1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 171kDa
Purification:
Affinity purification
Research Area:
Cancer, Signal Transduction
Swissprot #:
Q96QB1
User NOte:
Optimal dilutions for each application to be determined by the researcher.