ProSci

TFAM Antibody

Product Code:
 
PSI-18-390
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 2
Western blot analysis of extracts of various cell lines, using TFAM antibody (18-390) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
2 / 2
Immunofluorescence analysis of U2OS cells using TFAM antibody (18-390). Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using TFAM antibody (18-390) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunofluorescence analysis of U2OS cells using TFAM antibody (18-390). Blue: DAPI for nuclear staining.

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PSI-18-390-50uL50uL£433.00
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Further Information

Additional Names:
Transcription Factor AntibodiesAM, TCF6, MTTF1, MTTFA, TCF6L1, TCF6L2, TCF6L3
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:200
Background:
This gene encodes a key mitochondrial transcription factor containing two high mobility group motifs. The encoded protein also functions in mitochondrial DNA replication and repair. Sequence polymorphisms in this gene are associated with Alzheimer's and Parkinson's diseases. There are pseudogenes for this gene on chromosomes 6, 7, and 11. Alternative splicing results in multiple transcript variants.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human TFAM (NP_003192.1).
NCBI Gene ID #:
7019
NCBI Official Name:
transcription factor A, mitochondrial
NCBI Official Symbol:
TFAM
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 22kDa
Purification:
Affinity purification
Research Area:
Cancer, Cell Cycle, Neuroscience, Signal Transduction, Transcription
Swissprot #:
Q00059
User NOte:
Optimal dilutions for each application to be determined by the researcher.