ProSci

U2AF2 Antibody

Product Code:
 
PSI-18-399
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 3
Western blot analysis of extracts of various cell lines, using U2AF2 antibody (18-399) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
2 / 3
Immunofluorescence analysis of U2OS cells using U2AF2 antibody (18-399). Blue: DAPI for nuclear staining.
3 / 3
Immunoprecipitation analysis of 200ug extracts of SW620 cells using 1ug U2AF2 antibody (18-399). Western blot was performed from the immunoprecipitate using U2AF2 antibody (18-399) at a dilition of 1:500.
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Western blot analysis of extracts of various cell lines, using U2AF2 antibody (18-399) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunofluorescence analysis of U2OS cells using U2AF2 antibody (18-399). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of SW620 cells using 1ug U2AF2 antibody (18-399). Western blot was performed from the immunoprecipitate using U2AF2 antibody (18-399) at a dilition of 1:500.

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PSI-18-399-50uL50uL£433.00
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Further Information

Additional Names:
U2AF2, U2AF65
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:100

IP: 1:50 - 1:200
Background:
U2 auxiliary factor (U2AF), comprised of a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the U2AF large subunit which contains a sequence-specific RNA-binding region with 3 RNA recognition motifs and an Arg/Ser-rich domain necessary for splicing. The large subunit binds to the polypyrimidine tract of introns early during spliceosome assembly. Multiple transcript variants have been detected for this gene, but the full-length natures of only two have been determined to date.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 192-471 of human U2AF2 (NP_001012496.1).
NCBI Gene ID #:
11338
NCBI Official Name:
U2 small nuclear RNA auxiliary factor 2
NCBI Official Symbol:
U2AF2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 54kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
P26368
User NOte:
Optimal dilutions for each application to be determined by the researcher.