EXO1 Antibody

ProSci
Product Code: PSI-18-402
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-18-402-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using EXO1 Antibody (18-402) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
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Immunohistochemistry of paraffin-embedded human esophageal cancer using EXO1 antibody (18-402) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human esophagus using EXO1 antibody (18-402) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of U2OS cells using EXO1 antibody (18-402) at dilution of 1:100. Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using EXO1 Antibody (18-402) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunohistochemistry of paraffin-embedded human esophageal cancer using EXO1 antibody (18-402) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human esophagus using EXO1 antibody (18-402) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using EXO1 antibody (18-402) at dilution of 1:100. Blue: DAPI for nuclear staining.

Further Information

Additional Names:
EXO1, exonuclease 1, HEX1, hExoI, rad2 nuclease family member, homolog of S. cerevisiae exonuclease 1, EXOI
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100

IF: 1:50 - 1:200
Background:
This gene encodes a protein with 5' to 3' exonuclease activity as well as an RNase H activity. It is similar to the Saccharomyces cerevisiae protein Exo1 which interacts with Msh2 and which is involved in mismatch repair and recombination. Alternative splicing of this gene results in three transcript variants encoding two different isoforms.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 601-846 of human EXO1 (NP_006018.4).
NCBI Gene ID #:
9156
NCBI Official Name:
exonuclease 1
NCBI Official Symbol:
EXO1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 115kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
Q9UQ84
User NOte:
Optimal dilutions for each application to be determined by the researcher.