ProSci

TCP1 Antibody

Product Code:
 
PSI-18-409
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 3
Western blot analysis of extracts of various cell lines, using TCP1 antibody (18-409) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
2 / 3
Immunohistochemistry of paraffin-embedded rat kidney using TCP1 antibody (18-409) at dilution of 1:100 (40x lens).
3 / 3
Immunofluorescence analysis of U2OS cells using TCP1 antibody (18-409). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using TCP1 antibody (18-409) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded rat kidney using TCP1 antibody (18-409) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using TCP1 antibody (18-409). Blue: DAPI for nuclear staining.

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PSI-18-409-50uL50uL£433.00
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This product comes from: United States.
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Further Information

Additional Names:
TCP-1, t-complex 1, CCT-alpha, CCT1, CCTa, D6S230E, TCP-1-alpha, T-complex protein 1, alpha subunit, tailless complex polypeptide 1, CCTA
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
The protein encoded by this gene is a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Alternate transcriptional splice variants of this gene, encoding different isoforms, have been characterized. In addition, three pseudogenes that appear to be derived from this gene have been found.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 307-556 of human TCP1 (NP_110379.2).
NCBI Gene ID #:
6950
NCBI Official Name:
t-complex 1
NCBI Official Symbol:
TCP1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 60kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Signal Transduction
Swissprot #:
P17987
User NOte:
Optimal dilutions for each application to be determined by the researcher.