ProSci

UBE2D1 Antibody

Product Code:
 
PSI-18-410
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
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Western blot analysis of extracts of various cell lines, using UBE2D1 antibody (18-410) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.
2 / 4
Immunohistochemistry of paraffin-embedded rat brain using UBE2D1 antibody (18-410) at dilution of 1:100 (40x lens).
3 / 4
Immunohistochemistry of paraffin-embedded rat spleen using UBE2D1 antibody (18-410) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse spleen using UBE2D1 antibody (18-410) at dilution of 1:100 (40x lens).
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Western blot analysis of extracts of various cell lines, using UBE2D1 antibody (18-410) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.
Immunohistochemistry of paraffin-embedded rat brain using UBE2D1 antibody (18-410) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat spleen using UBE2D1 antibody (18-410) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spleen using UBE2D1 antibody (18-410) at dilution of 1:100 (40x lens).

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PSI-18-410-50uL50uL£433.00
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Further Information

Additional Names:
UBE2D1, E2(17)KB1, SFT, UBC4/5, UBCH5, UBCH5A
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200
Background:
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is closely related to a stimulator of iron transport (SFT), and is up-regulated in hereditary hemochromatosis. It also functions in the ubiquitination of the tumor-suppressor protein p53 and the hypoxia-inducible transcription factor HIF1alpha by interacting with the E1 ubiquitin-activating enzyme and the E3 ubiquitin-protein ligases. Two transcript variants encoding different isoforms have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-147 of human UBE2D1 (NP_003329.1).
NCBI Gene ID #:
7321
NCBI Official Name:
ubiquitin-conjugating enzyme E2D 1
NCBI Official Symbol:
UBE2D1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 14kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Immunology
Swissprot #:
P51668
User NOte:
Optimal dilutions for each application to be determined by the researcher.