ProSci

Histone H2B Antibody

Product Code:
 
PSI-18-414
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 4
Western blot analysis of extracts of various cell lines, using Histone H2B antibody (18-414) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
2 / 4
Immunohistochemistry of paraffin-embedded human esophagus using Histone H2B antibody (18-414) at dilution of 1:100 (40x lens).
3 / 4
Immunohistochemistry of paraffin-embedded rat kidney using Histone H2B antibody (18-414) at dilution of 1:100 (40x lens).
4 / 4
Immunohistochemistry of paraffin-embedded mouse liver using Histone H2B antibody (18-414) at dilution of 1:100 (40x lens).
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Western blot analysis of extracts of various cell lines, using Histone H2B antibody (18-414) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded human esophagus using Histone H2B antibody (18-414) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using Histone H2B antibody (18-414) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using Histone H2B antibody (18-414) at dilution of 1:100 (40x lens).

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PSI-18-414-50uL50uL£440.00
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Further Information

Additional Names:
H2B, H2BQ, GL105, H2B.1, H2BFQ, H2BGL105
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200
Background:
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-dependent histone that is a member of the histone H2B family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. The protein has antibacterial and antifungal antimicrobial activity.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-126 of human HIST2H2BE (NP_003519.1).
NCBI Gene ID #:
8349
NCBI Official Name:
histone cluster 2, H2be
NCBI Official Symbol:
HIST2H2BE
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 15kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
Q16778
User NOte:
Optimal dilutions for each application to be determined by the researcher.