ProSci

TAPBP Antibody

Product Code:
 
PSI-18-418
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 4
Western blot analysis of extracts of HT-29 cells, using TAPBP antibody (18-418) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
2 / 4
Immunohistochemistry of paraffin-embedded rat testis using TAPBP Antibody (18-418) at dilution of 1:200 (40x lens).
3 / 4
Immunohistochemistry of paraffin-embedded human gastric cancer using TAPBP Antibody (18-418) at dilution of 1:200 (40x lens).
4 / 4
Immunohistochemistry of paraffin-embedded mouse kidney using TAPBP Antibody (18-418) at dilution of 1:200 (40x lens).
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Western blot analysis of extracts of HT-29 cells, using TAPBP antibody (18-418) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat testis using TAPBP Antibody (18-418) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using TAPBP Antibody (18-418) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using TAPBP Antibody (18-418) at dilution of 1:200 (40x lens).

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PSI-18-418-50uL50uL£433.00
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Further Information

Additional Names:
TAPBP, NGS17, TAPA, TPN, TPSN
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100

IF: 1:50 - 1:100
Background:
This gene encodes a transmembrane glycoprotein which mediates interaction between newly assembled major histocompatibility complex (MHC) class I molecules and the transporter associated with antigen processing (TAP), which is required for the transport of antigenic peptides across the endoplasmic reticulum membrane. This interaction is essential for optimal peptide loading on the MHC class I molecule. Up to four complexes of MHC class I and this protein may be bound to a single TAP molecule. This protein contains a C-terminal double-lysine motif (KKKAE) known to maintain membrane proteins in the endoplasmic reticulum. This gene lies within the major histocompatibility complex on chromosome 6. Alternative splicing results in three transcript variants encoding different isoforms.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 30-260 of human TAPBP (NP_757345.2).
NCBI Gene ID #:
6892
NCBI Official Name:
TAP binding protein (tapasin)
NCBI Official Symbol:
TAPBP
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 48kDa
Purification:
Affinity purification
Research Area:
Immunology, Signal Transduction
Swissprot #:
O15533
User NOte:
Optimal dilutions for each application to be determined by the researcher.