ProSci

AP2B1 Antibody

Product Code:
 
PSI-18-434
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 4
Western blot analysis of extracts of various cell lines, using AP2B1 antibody (18-434) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
2 / 4
Immunohistochemistry of paraffin-embedded human esophagus using AP2B1 Antibody (18-434) at dilution of 1:100 (40x lens).
3 / 4
Immunohistochemistry of paraffin-embedded mouse lung using AP2B1 Antibody (18-434) at dilution of 1:100 (40x lens).
4 / 4
Immunohistochemistry of paraffin-embedded mouse stomach using AP2B1 Antibody (18-434) at dilution of 1:100 (40x lens).
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Western blot analysis of extracts of various cell lines, using AP2B1 antibody (18-434) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunohistochemistry of paraffin-embedded human esophagus using AP2B1 Antibody (18-434) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using AP2B1 Antibody (18-434) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse stomach using AP2B1 Antibody (18-434) at dilution of 1:100 (40x lens).

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PSI-18-434-50uL50uL£433.00
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Further Information

Additional Names:
AP2B1, ADTB2, AP105B, AP2-BETA, CLAPB1, DKFZp781K0743
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200
Background:
The protein encoded by this gene is one of two large chain components of the assembly protein complex 2, which serves to link clathrin to receptors in coated vesicles. The encoded protein is found on the cytoplasmic face of coated vesicles in the plasma membrane. Two transcript variants encoding different isoforms have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 752-951 of human AP2B1 (NP_001025177.1).
NCBI Gene ID #:
163
NCBI Official Name:
adaptor-related protein complex 2, beta 1 subunit
NCBI Official Symbol:
AP2B1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 105kDa
Purification:
Affinity purification
Research Area:
Neuroscience, Signal Transduction
Swissprot #:
P63010
User NOte:
Optimal dilutions for each application to be determined by the researcher.