NT5E / CD73 Antibody

ProSci
Product Code: PSI-18-454
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-18-454-50uL50uL£440.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using NT5E / CD73 antibody (18-454) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
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Immunohistochemistry of paraffin-embedded human liver using NT5E / CD73 antibody (18-454) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse testis using NT5E / CD73 antibody (18-454) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded rat testis using NT5E / CD73 antibody (18-454) at dilution of 1:100 (40x lens).

Western blot analysis of extracts of various cell lines, using NT5E / CD73 antibody (18-454) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunohistochemistry of paraffin-embedded human liver using NT5E / CD73 antibody (18-454) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse testis using NT5E / CD73 antibody (18-454) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat testis using NT5E / CD73 antibody (18-454) at dilution of 1:100 (40x lens).

Further Information

Additional Names:
NT5E, 5-nucleotidase, ecto (CD73), 5'-nucleotidase, 5 nucleotidase (CD73), 5-NT, 5-nucleotidase, 5'-nucleotidase, ecto (CD73), CD73, CD73 antigen, E5NT, Ecto-5-nucleotidase, ENT, EN, NT, Purine 5-Prime-Nucleotidase, NT5, NTE
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
The protein encoded by this gene is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. The encoded protein is used as a determinant of lymphocyte differentiation. Defects in this gene can lead to the calcification of joints and arteries. Two transcript variants encoding different isoforms have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 290-550 of human NT5E / CD73 (NP_002517.1).
NCBI Gene ID #:
4907
NCBI Official Name:
5'-nucleotidase, ecto (CD73)
NCBI Official Symbol:
NT5E
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 69kDa/75kDa
Purification:
Affinity purification
Research Area:
Immunology, Stem Cell
Swissprot #:
P21589
User NOte:
Optimal dilutions for each application to be determined by the researcher.