CDKN3 Antibody

ProSci
Product Code: PSI-18-472
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-18-472-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

1 / 2
Western blot analysis of extracts of mouse liver, using CDKN3 antibody (18-472) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
2 / 2
Immunofluorescence analysis of MCF-7 cells using CDKN3 antibody (18-472). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of mouse liver, using CDKN3 antibody (18-472) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunofluorescence analysis of MCF-7 cells using CDKN3 antibody (18-472). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
CDKN3, CDI1, CIP2, FLJ25787, KAP, KAP1, MGC70625
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
The protein encoded by this gene belongs to the dual specificity protein phosphatase family. It was identified as a cyclin-dependent kinase inhibitor, and has been shown to interact with, and dephosphorylate CDK2 kinase, thus prevent the activation of CDK2 kinase. This gene was reported to be deleted, mutated, or overexpressed in several kinds of cancers. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-172 of human CDKN3 (NP_001124323.1).
NCBI Gene ID #:
1033
NCBI Official Name:
cyclin-dependent kinase inhibitor 3
NCBI Official Symbol:
CDKN3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 23kDa
Purification:
Affinity purification
Research Area:
Cancer, Cell Cycle, Immunology, Signal Transduction
Swissprot #:
Q16667
User NOte:
Optimal dilutions for each application to be determined by the researcher.