GSK3B Antibody

ProSci
Product Code: PSI-18-485
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-18-485-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of mouse brain, using GSK3B antibody (18-485) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded human mammary cancer using GSK3B antibody (18-485) at dilution of 1:200 (40x lens).
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Immunohistochemistry of paraffin-embedded human liver cancer using GSK3B antibody (18-485) at dilution of 1:200 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse spleen using GSK3B antibody (18-485) at dilution of 1:200 (40x lens).
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Immunofluorescence analysis of C6 cells using GSK3B antibody (18-485) at dilution of 1:100. Blue: DAPI for nuclear staining.

Western blot analysis of extracts of mouse brain, using GSK3B antibody (18-485) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human mammary cancer using GSK3B antibody (18-485) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using GSK3B antibody (18-485) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spleen using GSK3B antibody (18-485) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of C6 cells using GSK3B antibody (18-485) at dilution of 1:100. Blue: DAPI for nuclear staining.

Further Information

Additional Names:
GSK3B, GSK-3 beta, GSK3 beta, GSK3beta, GSK3beta isoform, TPKI, GSK 3beta, Tau protein kinase I, ZGSK-3beta
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200

IP: 1:50 - 1:200
Background:
The protein encoded by this gene is a serine-threonine kinase, belonging to the glycogen synthase kinase subfamily. It is involved in energy metabolism, neuronal cell development, and body pattern formation. Polymorphisms in this gene have been implicated in modifying risk of Parkinson disease, and studies in mice show that overexpression of this gene may be relevant to the pathogenesis of Alzheimer disease. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A synthetic peptide of human GSK3B
NCBI Gene ID #:
2932
NCBI Official Name:
glycogen synthase kinase 3 beta
NCBI Official Symbol:
GSK3B
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 46kDa
Purification:
Affinity purification
Research Area:
Apoptosis, Cancer, Cell Cycle, Immunology, Neuroscience, Signal Transduction, Stem Cell
Swissprot #:
P49841
User NOte:
Optimal dilutions for each application to be determined by the researcher.