Ki67 Antibody

ProSci
Product Code: PSI-18-495
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-18-495-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using Ki67 antibody (18-495) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
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Immunohistochemistry of paraffin-embedded human lung cancer using Ki67 antibody (18-495) at dilution of 1:200 (40x lens).
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Immunofluorescence analysis of HeLa cells using Ki67 antibody (18-495) at dilution of 1:100. Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using Ki67 antibody (18-495) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
Immunohistochemistry of paraffin-embedded human lung cancer using Ki67 antibody (18-495) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of HeLa cells using Ki67 antibody (18-495) at dilution of 1:100. Blue: DAPI for nuclear staining.

Further Information

Additional Names:
monoclonal Ki-67, Proliferation-related Ki-67 antigen, Ki-67, KIA, HGNC:7107, antigen identified by monoclonal Ki-67, MKI67, MIB-1
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100

IF: 1:50 - 1:100
Background:
This gene encodes a nuclear protein that is associated with and may be necessary for cellular proliferation. Alternatively spliced transcript variants have been described. A related pseudogene exists on chromosome X.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1200-1300 of human Ki67 (NP_001139438.1).
NCBI Gene ID #:
4288
NCBI Official Name:
marker of proliferation Ki-67
NCBI Official Symbol:
MKI67
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 360kDa
Purification:
Affinity purification
Research Area:
Cancer, Cell Cycle, Neuroscience
Swissprot #:
P46013
User NOte:
Optimal dilutions for each application to be determined by the researcher.