MMP9 Antibody, KO Validated

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ProSci
Product Code: PSI-18-496
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-18-496-50uL50uL£505.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using MMP9 antibody (18-496) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
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Western blot analysis of extracts from normal (control) and MMP9 knockout (KO) 293T cells, using MMP9 antibody (18-496) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
3 / 3
Immunofluorescence analysis of U-2OS cells using MMP9 antibody (18-496) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using MMP9 antibody (18-496) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
Western blot analysis of extracts from normal (control) and MMP9 knockout (KO) 293T cells, using MMP9 antibody (18-496) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunofluorescence analysis of U-2OS cells using MMP9 antibody (18-496) at dilution of 1:100. Blue: DAPI for nuclear staining.

Documents

Further Information

Additional Names:
MMP9 Antibody : GELB, CLG4B, MMP-9, MANDP2, Matrix metalloproteinase-9, 92 kDa gelatinase
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:200
Background:
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 538-707 of human MMP9 (NP_004985.2).
NCBI Gene ID #:
4318
NCBI Official Name:
matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)
NCBI Official Symbol:
MMP9
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 110kDa
Purification:
Affinity purification
Research Area:
Apoptosis, Cancer, Cell Cycle, Signal Transduction
Swissprot #:
P14780
User NOte:
Optimal dilutions for each application to be determined by the researcher.
VALIDATION:
Antibody is Knockout validated.