SYK Antibody

ProSci
Product Code: PSI-18-517
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-18-517-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using SYK antibody (18-517) at 1:500 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
2 / 5
Immunohistochemistry of paraffin-embedded mouse brain using SYK Antibody (18-517) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded rat heart using SYK Antibody (18-517) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human stomach using SYK Antibody (18-517) at dilution of 1:100 (40x lens).
5 / 5
Immunofluorescence analysis of U2OS cells using SYK antibody (18-517) at dilution of 1:100. Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using SYK antibody (18-517) at 1:500 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded mouse brain using SYK Antibody (18-517) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using SYK Antibody (18-517) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using SYK Antibody (18-517) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using SYK antibody (18-517) at dilution of 1:100. Blue: DAPI for nuclear staining.

Further Information

Additional Names:
SYK, spleen tyrosine kinase, HGNC:11491, p72-Syk
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100

IF: 1:50 - 1:200
Background:
This gene encodes a member of the family of non-receptor type Tyr protein kinases. This protein is widely expressed in hematopoietic cells and is involved in coupling activated immunoreceptors to downstream signaling events that mediate diverse cellular responses, including proliferation, differentiation, and phagocytosis. It is thought to be a modulator of epithelial cell growth and a potential tumour suppressor in human breast carcinomas. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant protein of human SYK
NCBI Gene ID #:
6850
NCBI Official Name:
spleen tyrosine kinase
NCBI Official Symbol:
SYK
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 72KDA
Purification:
Affinity purification
Research Area:
Immunology, Innate Immunity, Signal Transduction
Swissprot #:
P43405
User NOte:
Optimal dilutions for each application to be determined by the researcher.