THY1 Antibody

ProSci
Product Code: PSI-18-520
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-18-520-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using THY1 antibody (18-520) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.
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Immunofluorescence analysis of Mouse brain cells using THY1 antibody (18-520) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of Rat brain cells using THY1 antibody (18-520) at dilution of 1:100. Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using THY1 antibody (18-520) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.
Immunofluorescence analysis of Mouse brain cells using THY1 antibody (18-520) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of Rat brain cells using THY1 antibody (18-520) at dilution of 1:100. Blue: DAPI for nuclear staining.

Further Information

Additional Names:
Thy-1 membrane glycoprotein, CDw90, Thy-1 antigen, CD90, THY1
Application Note:
WB: 1:1000 - 1:2000

IF: 1:50 - 1:200
Background:
This gene encodes a cell surface glycoprotein and member of the immunoglobulin superfamily of proteins. The encoded protein is involved in cell adhesion and cell communication in numerous cell types, but particularly in cells of the immune and nervous systems. The encoded protein is widely used as a marker for hematopoietic stem cells. This gene may function as a tumor suppressor in nasopharyngeal carcinoma. Alternative splicing results in multiple transcript variants.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 20-130 of human THY1 (NP_006279.2).
NCBI Gene ID #:
7070
NCBI Official Name:
Thy-1 cell surface antigen
NCBI Official Symbol:
THY1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 25kDa
Purification:
Affinity purification
Research Area:
Immunology, Neuroscience, Stem Cell
Swissprot #:
P04216
User NOte:
Optimal dilutions for each application to be determined by the researcher.